Geminal dihalogen isosteric replacement in hydrated AI-2 affords potent quorum sensing modulators.

نویسندگان

  • Min Guo
  • Yue Zheng
  • Jessica L Terell
  • Michal Ad
  • Clement Opoku-Temeng
  • William E Bentley
  • Herman O Sintim
چکیده

Hydrated carbonyl groups in AI-2, a quorum sensing autoinducer, make key hydrogen bonding interactions in the binding site of LsrR (a transcriptional regulator). This can be recapitulated with geminal dibromides, via halogen bonding. Geminal dihalogens represent interesting isosteric replacements for hydrated carbonyls in ligands and are currently under-utilized in ligand design.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Structure-Based Design and Biological Evaluation of Triphenyl Scaffold-Based Hybrid Compounds as Hydrolytically Stable Modulators of a LuxR-Type Quorum Sensing Receptor

Many common bacterial pathogens utilize quorum sensing to coordinate group behaviors and initiate virulence at high cell densities. The use of small molecules to block quorum sensing provides a means of abrogating pathogenic phenotypes, but many known quorum sensing modulators have limitations, including hydrolytic instability and displaying non-monotonic dose curves (indicative of additional t...

متن کامل

Development of Bacterial Quorum Sensing Inhibitors and Molecular Probes

Bacterial quorum sensing is regarded as a novel target for the design of antimicrobials. Based on lead structures identified from HTS, 39 analogues have been synthesized and evaluated in Vibrio haveyi. Potent inhibitors with IC50 values at single‐digit micromolar concentrations for AI‐2 mediated quorum sensing have been identified. On the second project, post‐synthesis modifications of DNA p...

متن کامل

Enhanced biofilm formation and reduced virulence of Actinobacillus pleuropneumoniae luxS mutant.

Actinobacillus pleuropneumoniae is the pathogen of porcine contagious pleuropneumonia causing great economic losses globally. LuxS is known to be involved in the interspecies quorum sensing by mediating the production of AI-2 signal molecule and play important roles in regulating various behaviors of bacteria. In this study, A. pleuropneumoniae was tested to be able to produce AI-2 like molecul...

متن کامل

DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli.

Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, a process known as quorum sensing. While the discovery of a diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several quorum sensing genes, the overall number and coordination of genes controlled by ...

متن کامل

ABSTRACT Title of dissertation: AUTOINDUCER-2 (AI-2) MEDIATED QUORUM SENSING IN ESCHERICHIA COLI

Title of dissertation: AUTOINDUCER-2 (AI-2) MEDIATED QUORUM SENSING IN ESCHERICHIA COLI Liang Wang, Doctor of Philosophy, 2004 Dissertation directed by: Professor William E. Bentley, Department of Chemical Engineering & Professor Steven W. Hutcheson, Department of Cell Biology and Molecular Genetics Bacteria have evolved complex genetic circuits to regulate their physiological activities and be...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Chemical communications

دوره 51 13  شماره 

صفحات  -

تاریخ انتشار 2015